In October, Uber lost another case when a tribunal ruled that it had to provide two of its drivers with at least minimum wage and also holiday pay.
This time Uber was fighting against proposals from public body Transport for London (TfL) which wants to enforce a standard for English reading and writing skills for drivers that the company believes is too high.
High Court Judge John Mitting ruled that TfL is entitled to require private hire drivers demonstrate English language compliance.
"Writing an essay has nothing to do with communicating with passengers or getting them safely from A to B," Uber's General Manager in London Tom Elvidge said, reports the Reuters News Agency.
"We intend to appeal this unfair and disproportionate new rule."